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Improving phenotypic testing of AMR by development of sensitive screening assays for emerging resistances, and setting missing ECOFFs. (IMPART) One health EJP

Published 2018-05-30

Currently,antimicrobial resistance poses an increasing threat to human health. In the EUAction Plan Against the rising threats from Antimicrobial Resistance: Road Map(EU 2015) one of the topics was reinforced joint cross-border research toaddress AMR, e.g. the development of diagnostic tools for the detection ofbacteria and resistance markers and harmonization of monitoring.

Thisproposal includes four topics related to the development and harmonization ofphenotypic methods for detection of antimicrobial resistance: (1) selectiveisolation and detection of colistin-resistant Enterobacteriaceae, (2) selectiveisolation and detection of carbapenemase-producing Enterobacteriaceae, (3)development of a standardized disk diffusion method for susceptibility testingof Clostridium difficile, (4) settingECOFFs for specific pathogen/antibiotic combinations.

Thisproject will result in a validated and sensitive method to detect colistin-resistant(carrying mcr) andcarbapenemase-producing Enterobacteriaceae (CPE) in caecal samples from animalsand in food. This is essential for the monitoring of the current prevalence andspread of bacteria carrying these emerging resistance genes in animals and therisk of transmission of these genes via food. Additionally, in order to providea suitable and cost-effective method for antimicrobial resistance surveillanceof the zoonotic pathogen C. difficile,a disk diffusion method will be developed and standardized for a broad range ofantimicrobials. The generated distributions of inhibition zone diameter willsupport future work on setting epidemiological cut-off values (ECOFFs). Furthermore,the setting of ECOFFs for veterinary pathogens will highly improve harmonizationof monitoring of resistance in animal pathogens and support the process fordefining animal species specific clinical breakpoints for veterinaryantimicrobials which will ultimately lead to more effective and prudent use ofantimicrobials in animals.